Root essential oil ofChrysopogon zizanioidesrelaxes rat isolated thoracic aorta – anex vivoapproach
| Autor: | Venkatraman Sriramavaratharajan, Ramar Murugan, David Raj Chellappan, Lekha Sivakumar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology medicine.drug_class Calcium channel 030302 biochemistry & molecular biology Potassium channel blocker Calcium channel blocker Pharmacology General Biochemistry Genetics and Molecular Biology law.invention Nitric oxide Nitric oxide synthase 03 medical and health sciences chemistry.chemical_compound chemistry law medicine.artery medicine biology.protein Thoracic aorta Phenylephrine Essential oil 030304 developmental biology medicine.drug |
| Zdroj: | Zeitschrift für Naturforschung C. 76:161-168 |
| ISSN: | 1865-7125 0939-5075 |
| DOI: | 10.1515/znc-2020-0143 |
| Popis: | Root ofChrysopogon zizanioides(L.) Roberty has been used in Siddha system of medicine to treat hypertension. The present study was therefore to investigate the vasorelaxation effect of root essential oil ofC. zizanioidesusing rat isolated thoracic aortic rings. Chemical characterization of root essential oil was carried out using Gas Chromatography–Flame Ionization Detector (GC–FID) and Gas Chromatography–Mass Spectrometry (GC–MS). Essential oil nanoemulsion (EONE) was prepared and characterized. Vasorelaxant effect of EONE in endothelium-intact aortic rings precontracted with phenylephrine (PE) (1 µM) or KCl (80 mM) was investigated. Role of Ca2+, nitric oxide and K+channels in precontracted aortic rings were investigated to elucidate the mechanism of action of the essential oil. Further, the role of muscarinic and prostacyclin receptors in EONE induced relaxation was studied. The EONE significantly induced relaxation (Emax77.1 ± 4.87%) in PE precontracted aortic rings. The nitric oxide synthase, and cyclooxygenase inhibitors and potassium channel blockers have not significantly inhibited the vasorelaxation induced by EONE. However, EONE induced relaxation in precontracted endothelium-intact aortic rings was significantly inhibited by muscarinic receptor and calcium channel. The root essential oil ofC. zizanioidespossesses vasorelaxant effect through muscarinic pathway as well as acts as calcium channel blocker. |
| Databáze: | OpenAIRE |
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