| Popis: |
Currently, nanoparticles (NPs) are in use in several applications especially in the biomedical field. The NPs showed promising results in the treatment of several diseases, however, NP S could have toxic effects on some vital organs. This study was conducted to evaluate the possible toxicity of Cobalt/Ferrite (CF NPs) and Cobalt/Zinc/Ferrite (CZF NPs) nano-complex. Eighty female mice were used to determine the median lethal dose (LD 50 ) of CF NPs (100 mg/kg) and CZF NPs (100 mg/kg). Thirty female mice CD1 were divided into 3 groups (n=10) as follows: Group 1 (Gp1). Mice were injected with 200 µl of sterile saline interperitoneal (i.p.). Gp2 and Gp3 were injected with CF NPs and CFZ NPs for 6 days. Hematological, biochemical, and histopathological parameters were determined at day 14 post injection. The results showed that there was a significant change in the total body weight in CZF NPs injected mice. CF NPs injections did not alter the values of the total red blood cells (RBCs), haemoglobin conc (Hb), hematocrit Hct%, total white blood cells (WBCs) and platelets while CZF NPs injections increased WBCs count and decrease the platelets count. Also, injection of CZF NPs altered the differential leukocyte percentages. There was no significant change in liver and kidney functions in CF NPs injected mice, while CZF NPs exhibited significant increases in liver and kidney bio-markers. The injection of mice with CF NP S didn´t alters the histological architecture of hepatic and renal tissues, while the hepatic and renal structures were disorganized in CZF NPs injected mice. Collectively, the results revealed that CZF NPs showed a degree of toxicity on experimental mice. |
