P1.07 Contribution of pcr in the biological diagnosis oftrichomonas vaginalisinfection at institut pasteur of cote d'ivoire
| Autor: | J Ouhon, Angora Ke, A Ouattara, Offianan At, A Ira Bonouman, H Faye Kette, Blavo Kouame Be, Alain Yeo |
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| Rok vydání: | 2017 |
| Předmět: |
biology
business.industry Direct examination biology.organism_classification medicine.disease_cause Virology Giemsa stain law.invention Microbiology Gram staining law Multiplex polymerase chain reaction medicine Neisseria gonorrhoeae Trichomonas vaginalis Mycoplasma genitalium Chlamydia trachomatis business |
| Zdroj: | Systems Biology and Novel Technologies For Molecular Analysis and Diagnosis. |
| DOI: | 10.1136/sextrans-2017-053264.115 |
| Popis: | Introduction The urogenital trichomonasis remains the most common non-viral sexually transmitted infection. Classically, its biological diagnosis relies on the use of microscopy which is often negative because of the fragility of the parasite. PCR is thus an alternative method. So, this study aims to show the importance of PCR in the diagnosis of Trichomonas vaginalis . Methods This cross-sectional study was conducted among 194 women received at the Institut Pasteur of Cote d’Ivoire from July to October 2013 for an STD through a vaginal sample with three swabs. Direct examination was made on each sample, Gram stain and Giemsa, culture as well as a multiplex PCR ( T. vaginalis , Chlamydia trachomatis , Neisseria gonorrhoeae and Mycoplasma genitalium ). The sociodemographic and clinical data were collected using a questionnaire. Results In total, 194 women were received with an average age of 31.35 years (SD=8.60 years). At microscopy, 2 cases (1.03%) of T. vaginalis were revealed. After DNA extraction, a PCR amplification has allowed to identify 7 cases (3, 61%) of T. vaginalis with a sensitivity of 100% and specificity of 97.4%. Other germs were identified by PCR, Chlamydia trachomatis (4.12%), Neisseria gonorrhoeae (2.58%) and Mycoplasma genitalium (1.03%). Conclusion Routine testing of Trichomonas vaginalis by PCR has shown the importance of this method in the diagnosis of Trichomonas vaginalis infection because of its high sensibility and specificity. Its might be an alternative after the initiation of classical microscopy. |
| Databáze: | OpenAIRE |
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