| Autor: |
Pierre Roques, Françoise Thomé, Martin Olomucki |
| Rok vydání: |
1988 |
| Předmět: |
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| Zdroj: |
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 951:71-77 |
| ISSN: |
0167-4781 |
| DOI: |
10.1016/0167-4781(88)90026-7 |
| Popis: |
The reaction of yeast tRNAPhe with methyl chlorotetrolate, ClCH2-C≡C-COOCH3, was studied. This reagent converts adenine and cytosine rings into derivatives in which an additional heterocycle bearing the alkylating chloromethyl group is fused to the original base; these derivatives can exist in two isomeric forms. Modified nucleosides of this type can be easily identified by reverse-phase HPLC. It was found that under native conditions, the modification of tRNA involves the anticodon loop and the 3′-end. The isomers of adenine derivatives formed in the anticodon loop were different from those formed in the 3′-end. It is suggested that the isomeric structure of the derivatives is related to the fine conformational differences between these two regions of tRNAPhe. Methyl chlorotetrolate could thus be used as a conformational probe of single-stranded nucleic acids. Preliminary assays showed that modified tRNAPhe binds irreversibly to yeast phenylalanyl-tRNA synthetase. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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