Combinatorial decoding of the invariantC. elegansembryonic lineage in space and time
| Autor: | John I. Murray, Amanda L. Zacharias |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Regulation of gene expression Cell type ved/biology Cellular differentiation ved/biology.organism_classification_rank.species Robustness (evolution) Cell Biology Computational biology Biology biology.organism_classification Embryonic stem cell Cell biology 03 medical and health sciences 030104 developmental biology Endocrinology Genetics Model organism Developmental biology Caenorhabditis elegans |
| Zdroj: | genesis. 54:182-197 |
| ISSN: | 1526-954X |
| DOI: | 10.1002/dvg.22928 |
| Popis: | Understanding how a single cell, the zygote, can divide and differentiate to produce the diverse animal cell types is a central goal of developmental biology research. The model organism Caenorhabditis elegans provides a system that enables a truly comprehensive understanding of this process across all cells. Its invariant cell lineage makes it possible to identify all of the cells in each individual and compare them across organisms. Recently developed methods automate the process of cell identification, allowing high-throughput gene expression characterization and phenotyping at single cell resolution. In this Review, we summarize the sequences of events that pattern the lineage including establishment of founder cell identity, the signaling pathways that diversify embryonic fate, and the regulators involved in patterning within these founder lineages before cells adopt their terminal fates. We focus on insights that have emerged from automated approaches to lineage tracking, including insights into mechanisms of robustness, context-specific regulation of gene expression, and temporal coordination of differentiation. We suggest a model by which lineage history produces a combinatorial code of transcription factors that act, often redundantly, to ensure terminal fate. |
| Databáze: | OpenAIRE |
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