Coenzyme production using immobilized enzymes. II. Factors affecting the continuous production of NADP

Autor: Bela Szajani, L.M. Simon, M. Kotormán
Rok vydání: 1994
Předmět:
Zdroj: Enzyme and Microbial Technology. 16:236-239
ISSN: 0141-0229
DOI: 10.1016/0141-0229(94)90048-5
Popis: The effects of certain factors on the continuous production of NADP+ with immobilized NAD+ kinase were studied. In a column reactor, the maximum NADP+ productivity was experienced at pH 8.0. The presence of 10–12 mM Mg2+ ion appeared to be optimum for maximum productivity. K+ ion at 1–5 mM behaved as an activator. Mn2+ and Zn2+ ions proved effective activators of the immobilized NAD+ kinase. Systems of up to three or four columns were most effective in NADP+ production.
Databáze: OpenAIRE