Coenzyme production using immobilized enzymes. II. Factors affecting the continuous production of NADP
Autor: | Bela Szajani, L.M. Simon, M. Kotormán |
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Rok vydání: | 1994 |
Předmět: | |
Zdroj: | Enzyme and Microbial Technology. 16:236-239 |
ISSN: | 0141-0229 |
DOI: | 10.1016/0141-0229(94)90048-5 |
Popis: | The effects of certain factors on the continuous production of NADP+ with immobilized NAD+ kinase were studied. In a column reactor, the maximum NADP+ productivity was experienced at pH 8.0. The presence of 10–12 mM Mg2+ ion appeared to be optimum for maximum productivity. K+ ion at 1–5 mM behaved as an activator. Mn2+ and Zn2+ ions proved effective activators of the immobilized NAD+ kinase. Systems of up to three or four columns were most effective in NADP+ production. |
Databáze: | OpenAIRE |
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