Synthesis and application of a potential therapeutic coumarin derivative against IHNV in aquaculture
| Autor: | Da-Wei Song, Lei Liu, Huan Wang, Li-Peng Shan, Yang Hu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
medicine.medical_treatment Intraperitoneal injection 04 agricultural and veterinary sciences Glutathione Aquatic Science Biology Malondialdehyde Molecular biology In vitro Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound chemistry In vivo 040102 fisheries medicine biology.protein 0401 agriculture forestry and fisheries Rainbow trout 030304 developmental biology Cytopathic effect |
| Zdroj: | Aquaculture. 543:736999 |
| ISSN: | 0044-8486 |
| Popis: | Infectious haematopoietic necrosis virus (IHNV) is one of the most serious pathogens in aquaculture, causing devastating damage to salmonid. In this study, a novel coumarin derivative (DC) was designed and synthesized to evaluate its anti-IHNV effects in vitro and in vivo. DC at the concentration of up to 10 mg/L significantly inhibited IHNV replication in epithelioma papulosum cyprini (EPC) cells with a maximum inhibitory rate of >95%. Additionally, the results revealed that DC treatment significantly suppressed the IHNV-induced cytopathic effect (CPE) in host cells. Moreover, further data found that DC significantly increased superoxide dismutase (SOD), glutathione (GSH) and total antioxidant capacity (T-AOC) activities, and decreased malondialdehyde (MDA) content in EPC cells and in rainbow trout, suggesting that IHNV-induced antioxidant-oxidant imbalance was inhibited by DC. For in vivo studies, DC exhibited an anti-IHNV effect by substantially enhancing the survival rate of virus-infected fish via intraperitoneal injection. Additionally, DC enhanced the levels of IFN-related genes demonstrated that DC indirectly activated IFNs for the clearance of IHNV in rainbow trout. Importantly, the data showed that horizontal transmission of IHNV was reduced by DC in a static cohabitation challenge model, especially for recipient fish in bath treatment, which suggested that DC might be suitable as a possible therapeutic agent for IHNV in aquaculture. |
| Databáze: | OpenAIRE |
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