A high sensitivity bead-based immunoassay with nanofluidic preconcentration for biomarker detection
| Autor: | Horn-Jiunn Sheen, Chih Zong Deng, Pei-Shan Chung, Yu Jui Fan, Wei-Cheng Tian |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry High selectivity Metals and Alloys 02 engineering and technology Mixed solution Velocimetry Bead 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials visual_art Immunoassay Materials Chemistry visual_art.visual_art_medium medicine Electrical and Electronic Engineering 0210 nano-technology Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 272:502-509 |
| ISSN: | 0925-4005 |
| Popis: | In this study, we developed a rapid biomarker sensor with a high sensitivity and high selectivity by applying a bead-based immunosensing technique in a nanofluidic preconcentration device. Antibody-coated 300-nm beads and antigens were mixed and then pumped into a valve-integrated nanofluidic preconcentration device for collection and trapping. Because of size differences between the molecules and 300-nm beads, the antigen (molecule) in the mixed solution could be concentrated by up to 10,000-fold in 2 min, but the nanobeads could not be thus concentrated. After concentrated antigens and antibody-coated nanobeads were isolated, micro-particle tracking velocimetry was used to measure the Brownian diffusion of the nanobeads in real time to determine the antigen concentration. Through this preconcentration experiment, we demonstrated a rapid and high-sensitivity detection of prostate-specific antigen with a detection limit of 50 pg/ml in 20 min. |
| Databáze: | OpenAIRE |
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