Chronic THC vapor inhalation rescues hyperalgesia in rats with chronic inflammation and produces sex-specific alterations in midbrain neuronal activity
| Autor: | Matthew N. Hill, Jason W. Middleton, Nicholas W. Gilpin, S. H. Lightfoot, Leslie K Kelley |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Inhalation
business.industry organic chemicals medicine.medical_treatment Chronic pain Pharmacology Neurotransmission medicine.disease Inhibitory postsynaptic potential Periaqueductal gray DAMGO chemistry.chemical_compound chemistry Opioid mental disorders Medicine Cannabinoid business medicine.drug |
| DOI: | 10.1101/2021.09.23.461562 |
| Popis: | In an effort to reduce reliance on opioids for the treatment of pain in the clinic, ongoing work is testing the utility of cannabinoid drugs as a potential alternative for treatment of chronic pain. We tested chronic THC vapor inhalation effects on thermal nociception and mechanical sensitivity, as well as midbrain (i.e., ventrolateral periaqueductal gray [vlPAG]) neuronal function, in adult male and female Wistar rats with chronic inflammatory pain (CFA treatment). We report that chronic THC vapor inhalation rescues both thermal hyperalgesia and mechanical hypersensitivity in males treated with CFA, but only thermal hyperalgesia in CFA females. Most of the anti-hyperalgesic effects of chronic THC vapor were still observable 24 hours after cessation of the last THC exposure. We also report that chronic THC vapor inhalation modulates intrinsic and synaptic properties of vlPAG neurons, including reductions in action potential firing rate and spontaneous inhibitory synaptic transmission in males, and that these effects occur specifically in neurons that respond to current input with a “delayed” firing phenotype. Treatment with CFA led to increased firing rate and increased sIPSC amplitude in vlPAG neurons of female rats, and chronic THC vapor rescued sIPSC amplitudes to control levels – these effects were specific to vlPAG neurons categorized as having an “onset” firing phenotype. Ongoing work is exploring sex-specific mechanisms (e.g., CB1 receptor) of THC vapor rescue effects in the vlPAG of rats treated with CFA, and further exploring the vlPAG cell types impacted by CFA treatment and chronic THC vapor inhalation.Significance StatementMany adults in the U.S. with pain self-medicate with THC and cannabis, and many humans use e-cigarette type devices filled with cannabis extracts to self-administer THC and other constituents of the marijuana plant. Until recently, most rodent studies of THC effects on brain and behavior have used injection procedures and male rats. Here, we tested the effect of chronic THC vapor inhalation on pain-related behaviors and midbrain neural circuit function in adult male and female Wistar rats. As predicted, chronic THC vapor inhalation rescued chronic inflammatory pain effects on behavior and midbrain neuronal function. |
| Databáze: | OpenAIRE |
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