Macrophage Persistence in Adipose Tissue of Rhesus Macaques

Autor: Marissa Fahlberg, Xavier Alvarez, Cecily Midkiff, Elizabeth Didier, Marcelo J Kuroda
Rok vydání: 2017
Předmět:
Zdroj: The Journal of Immunology. 198:125.11-125.11
ISSN: 1550-6606
0022-1767
Popis: BACKGROUND HIV/SIV establishes lifelong infection by integrating proviral DNA into the genome of susceptible long-lived immune cells. In order to eliminate the virus, cellular reservoirs must be removed. Long-lived macrophages are permissible to SIV infection and replication. Given recent publications describing adipose as a tissue reservoir for HIV in HAART-treated patients, we chose to investigate the abundance of macrophages in adipose tissue and the proportion that are long-lived to determine their potential as viral reservoirs. METHODS Mesenteric adipose tissue from three RM infected with SIVmac239 intravenously and three uninfected RM were analyzed by confocal microscopy to determine frequency of CD163+ macrophages among nucleated cells. An additional two macaques (one SIVmac239-infected, one uninfected) were injected with dextran intravenously, an inert molecule which can be phagocytosed by macrophages and detected at later time points. Tissues were analyzed by IHC and flow cytometry. RESULTS CD163+ macrophages constituted 10.5% ± 2.0 of all nucleated cells in the adipose of three uninfected macaques, which differed significantly from infected macaques where macrophages made up 22.0% ± 3.1 of all nucleated cells. We detected dextran in cells within adipose tissue from both injected rhesus macaques at the time of death. The time points at which they died were 43 days (uninfected animal) and 50 days (infected animal) post-injection. Macrophages constitute a significant proportion of nucleated cells in adipose tissue of both uninfected and infected macaques, and their incorporation of dextran is indicative of a long-lived tissue-resident macrophage subtype with potential to be a reservoir for HIV/SIV.
Databáze: OpenAIRE
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