Morphological basis for possibility of applying electrochemical method with use of nanotechnological biosensors in diagnosis of colorectal cancer
| Autor: | A. G. Kochetov, G. G. Freind, A. N. Belkin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty business.industry Colorectal cancer Mean value Colon mucosa medicine.disease Tumor tissue 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Current strength Medicine Alkaline phosphatase Immunohistochemistry business Biosensor |
| Zdroj: | Perm Medical Journal. 38:88-96 |
| ISSN: | 2687-1408 0136-1449 |
| DOI: | 10.17816/pmj38388-96 |
| Popis: | Objective. To assess the efficacy of electrochemical method using biosensors for studying the activity of alkaline phosphatase as a marker of functional atypism in the fresh and formalin-fixed biopsies of the colorectal cancer by means of comparing with the results of morphological study. Materials and methods. Electrochemical method was used to study the fragments of tumors (adenocarcinomas) of the colon and colon mucosa from 78 patients who underwent endoscopic study of the colon. The results of electrochemical and morphological studied were assessed and compared. Results. In the material from 70 patients, a mean value of the current strength, obtained while studying the tumor fragments, was 49.2 (95 % CI 41.3 88.9) nA and 33.1 (95 % CI 9.5 44.2) nA in case of fresh and formalin-fixed biopsies, respectively. A mean value of the current strength, obtained while studying mucosa fragments, was 119.7 (95 % CI 96.8 167.1) nА and 59.6 (95 % CI 48.3 71) nA in case of fresh and formalin-fixed biopsies, respectively. While conducting immunohistochemical study, in the tumor tissue a low expression of alkaline phosphatase compared to unchanged mucosa was detected. Conclusions. Electrochemical method applying nanotechnological biosensors was shown to be used for a rapid, direct estimation of alkaline phosphatase content in the biopsies of the colorectal carcinoma. The obtained results allow considering electrochemical method as an additional method for assessment of functional atypism of tumor cells. |
| Databáze: | OpenAIRE |
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