Abstract 450: Global Phosphopeptide Analyses Identifies a Profile that Distinguishes Advanced Heart Failure Patients Capable of Cardiac Recovery Following LVAD Unloading
| Autor: | Aman Makaju, Craig H. Selzman, Sutip Navankasattusas, Stavros G. Drakos, Lauren McCreath, Sarah Franklin, Christopher M. Tracy, Nikolaos A. Diakos |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Circulation Research. 123 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/res.123.suppl_1.450 |
| Popis: | The classical view of the human heart characterizes it as an organ with limited ability to recover and restore itself. Consequently, left ventricular assist devices (LVAD), which provide mechanical circulatory support and profound ventricular unloading, have been used as both a destination therapy as well as a bridge to transplantation in heart failure patients. However, evidence from many prospective studies indicates that a subset of patients with LVAD implants can significantly improve the structure and function of their heart (i.e. “responders”) and undergo LVAD explantation. This intriguing phenomenon provides great promise for heart failure patients, although the underlying mechanisms driving this recovery are largely unknown. To identify global changes in signaling pathways operative in the normal and failing human heart and to distinguish patients who respond to LVAD therapy from non-responders we performed phosphopeptide profiling and label-free quantitation of 38 patients. Our analysis utilized cardiac tissue from 10 donor controls, 6 recovered heart failure patients (responder), and 22 patients that did not respond to LVAD therapy (non-responders). Phosphopeptide enrichment and mass spectrometry-based analyses identified 15,816 unique phosphopeptides, 2,017 of which were shared between all samples. Label-free quantitation and bioinformatic analysis further classified 288 peptides [≥ 2 fold change (p |
| Databáze: | OpenAIRE |
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