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The speed, sensitivity and specificity of nucleic acid amplification techniques make them very attractive tools for diagnostic virology. Priorities should be defined for the stepwise implementation of these methods. Their application is not limited to the purpose of direct diagnosis, but includes quantitation of viral load, evaluation of genetic determinants of resistance to antiviral drugs, typing of strains, and the analysis of new viral pathogens. However, a widespread use of these techniques by clinical laboratories will require fully standardized, carefully controlled systems. Very few commercial systems are as yet available. |
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