Characterization and differentiation of Erwinia carotovora strains from mulberry trees

Autor:	Seo Sang-Tae, Kenichi Tsuchiya, Ritsuko Murakami
Rok vydání:	2004
Předmět:	Gel electrophoresis biology Molecular mass food and beverages Plant Science Erwinia biology.organism_classification Microbiology RAPD chemistry.chemical_compound chemistry Erwinia species Mulberry trees Agronomy and Crop Science Polyacrylamide gel electrophoresis DNA
Zdroj:	Journal of General Plant Pathology. 70:120-123
ISSN:	1610-739X 1345-2630
DOI:	10.1007/s10327-003-0098-z
Popis:	We recently reported that two diverse types (types 1 and 2) were identified among strains of Erwinia carotovora from mulberry trees. Type 1 strains were similar to E. carotovora subsp. carotovora (Ecc), whereas type 2 strains were distinct from Ecc and other E. carotovora strains. In this study, seven more mulberry strains of type 2 and reference strains were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and randomly amplified of polymorphic DNA (RAPD). On the basis of SDS-PAGE profiles of whole-cell proteins, type 2 strains had high similarity with one another. In addition, they had an unique peptide band with a molecular mass of approximately 28 kDa. RAPD analysis showed that they were also effectively differentiated by a strong, specific RAPD fragment for type 2 strains. Based on these two approaches, we have confirmed that the present type 2 strains from mulberry can be discriminated clearly from other soft rot Erwinia species.
Databáze:	OpenAIRE
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