Using chitosan-stabilized, hyaluronic acid-modified selenium nanoparticles to deliver CD44-targeted PLK1 siRNAs for treating bladder cancer

Autor:	Maryamsadat Shahidi, Omid Abazari, Parisa Dayati, Javad Zavar Reza, Mohammad Hossein Modarressi, Davood Tofighi, Bibi Fatemeh Haghiralsadat, Fatemeh Oroojalian
Rok vydání:	2023
Předmět:	Biomedical Engineering Medicine (miscellaneous) General Materials Science Bioengineering Development
Zdroj:	Nanomedicine. 18:259-277
ISSN:	1748-6963 1743-5889
DOI:	10.2217/nnm-2022-0198
Popis:	Aims: Achieving an effective biocompatible system for siRNAs delivery to the tumor site remains a significant challenge. Materials & methods: Selenium nanoparticles (SeNPs) modified by chitosan (CS) and hyaluronic acid (HA) were fabricated for PLK1 siRNAs ( siPLK1) delivery to the bladder cancer cells. The HA-CS-SeNP@siPLK1 efficacy was evaluated using in vitro and in vivo models. Results: HA-CS-SeNP@siPLK1 was selectively internalized into T24 cells through clathrin-mediated endocytosis. Treatment with HA-CS-SeNP@siPLK1 successfully silenced the PLK1 gene, inhibited cell proliferation and induced cell cycle arrest in vitro. HA-CS-SeNP@siPLK1 could also inhibit tumor growth in vivo without causing systemic toxicity. Conclusion: Our results suggest that HA-CS-SeNPs may provide a good vehicle for delivering siPLK1 to the bladder tumor site.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f0801257330169466e3e5db094501a32 https://doi.org/10.2217/nnm-2022-0198 Zobrazit plný text záznamu