| Popis: |
The inhibitory effect of buckwheat seed extract on the assay of glucose by glucose oxidase-peroxidase-chromogen methods was demonstrated. The inhibitory effect was caused by substances in the extract, and act as inhibitor on peroxidase system used in this enzymatic glucose assay system. The inhibitory effect is unchangeable by diastase digestion, heat, acid and alkali treatments. The inhibitory substances in buckwheat seed extract, which are absorbed by charcoal and eluted efficiently by 80% ethanol, were fractionated by gel-filtration on Sephadex G-10 column. The gel-filtration gave three active fractions, one of which showed about 11 times inhibitory power against that of starting material. |
