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102 Escherichia coli strains from patients with community-acquired urinary tract infections (UTIs) were genotypically and phenotipically characterized. The strains were examined genotypically by using the polymerase chain reaction (PCR) for presence of five urovirulence operons: α-haemolysin (hly), cytotoxic necrotizing factor type 1 (cnf1), pyelonephritis-associated pili (pap), S. fimbriae (sfa) and afimbrial adhesin (afa). The phenotypic methods used were: Vero and HeLa cells for detection of CNF1, blood agar for Hly and mannose-resistant haemagglutination typing for expression of adhesins. There was a very good correlation between the results obtained by PCR and those obtained by phenotypic methods. Thus, the PCR assays can by recommended for clinical use to detect uropathogenic E. coli strains, as well as for epidemiological studies. |
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