Removal of bisphenol A by laccases from Pleurotus ostreatus and Pleurotus pulmonarius and evaluation of ecotoxicity of degradation products
| Autor: | Adelar Bracht, Mariene Marques Nolli, Emanuelle Neiverth de Freitas, Gisele Adriana Bubna, Rosane Marina Peralta, Cristina Giatti Marques de Souza, Rosely A. Peralta, Regina de Fátima Peralta Muniz Moreira, Camila Gabriel Kato, Tatiane Brugnari, Thalita Grando Rauen |
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| Rok vydání: | 2017 |
| Předmět: |
endocrine system
Bisphenol A General Chemical Engineering Metabolite 02 engineering and technology 010501 environmental sciences 01 natural sciences Industrial and Manufacturing Engineering chemistry.chemical_compound Environmental Chemistry Organic chemistry Food science 0105 earth and related environmental sciences Laccase biology urogenital system Chemistry Pleurotus pulmonarius General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Endocrine disruptor Toxicity Pleurotus ostreatus Ecotoxicity 0210 nano-technology hormones hormone substitutes and hormone antagonists |
| Zdroj: | Chemical Engineering Journal. 330:1361-1369 |
| ISSN: | 1385-8947 |
| DOI: | 10.1016/j.cej.2017.08.051 |
| Popis: | Bisphenol A (BPA) is an endocrine disruptor compound, that is continuously released into the environment and is barely degraded in wastewater treatment plants. In this work, crude laccases from Pleurotus ostreatus and Pleurotus pulmonarius were used for the first time to degrade BPA in aqueous solution. The residual BPA was quantified by HPLC and the BPA metabolites produced by action of the laccases were identified by GC–MS. BPA at 100 mg/L and 200 mg/L (0.88 mmol/L) were 100% and 85% removed, respectively, in a 1 h reaction by both fungal laccases at a concentration of 8 U/L. Thirteen aromatic and aliphatic BPA metabolites were identified, including p-isopropenylphenol, methylpent-3-oic acid, ethyl-3-ethoxy propanoate, and 4-ethyl-2-methoxyphenol. The acute BPA toxicity decreased from 85% to less than 5% using the P. ostreatus laccase. On the other hand, P. pulmonarius laccase, did not caused reduction in toxicity, possibly because at least one BPA metabolite was as toxic as the parent compound itself. The results of this study suggest a feasible method for the complete removal of BPA from polluted environments using crude laccase from P. ostreatus. |
| Databáze: | OpenAIRE |
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