Micropropagation of Philodendron selloum: Influence of copper sulfate on endophytic bacterial contamination, antioxidant enzyme activity, electrolyte leakage, and plant survival
| Autor: | Mohammed Elsayed El-Mahrouk, Mayada Kadry Seliem, A. N. El-Banna, Yaser Hassan Dewir, Yaser M. Hafez |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
biology Plant tissue culture food and beverages Plant Science biology.organism_classification 01 natural sciences Polyphenol oxidase Enzyme assay 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Horticulture chemistry Micropropagation Catalase Shoot biology.protein Sulfate Bacteria 010606 plant biology & botany |
| Zdroj: | South African Journal of Botany. 139:230-240 |
| ISSN: | 0254-6299 |
| Popis: | Endophytic microorganisms is a major constrain to the establishment and growth of tissue culture plants. We report the use of copper sulfate (CuSO4.5H2O) to eliminate the endophytic bacteria in Philodendron selloum in vitro cultures. Contaminated shoots were cultured onto Murashige and Skoog (MS) medium containing 5 mg/L BA and supplemented with different concentrations of copper sulfate at 0, 35, 70 and 140 mg/L for 6 weeks. Copper sulfate at 70 mg/L completely eliminated the endogenous bacteria without decline in plant growth. However, 35 mg/L copper sulfate was optimal for maximum shoot multiplication (25), survival percentage (100%) and growth of plants. Antioxidant enzymes activity of catalase, peroxidases, and polyphenol oxidase were increased because of copper sulfate treatments. Conversely, electrolyte leakage was decreased at low copper sulfate (≤70 mg/L). Randomly amplified polymorphic DNA (RAPD) analysis revealed that plantlets exposed to different levels of copper sulfate were not genetically different from control plants. |
| Databáze: | OpenAIRE |
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