Heterologous expression of a synthetic gene encoding a novel hevein-type antimicrobial peptide of Leymus arenarius in Escherichia coli cells

Autor:	T. I. Odintsova, Eugene A. Rogozhin, E. V. Grishin, E. O. Zhabon, Ts. A. Egorov, V. A. Pukhalskiy, L. L. Utkina, Anna A. Slavokhotova, A. N. Shiyan
Rok vydání:	2010
Předmět:	chemistry.chemical_classification biology Target peptide Peptide medicine.disease_cause biology.organism_classification Microbiology chemistry Biochemistry Leymus arenarius Gene expression Genetics medicine Heterologous expression Thioredoxin Escherichia coli Gene
Zdroj:	Russian Journal of Genetics. 46:1449-1454
ISSN:	1608-3369 1022-7954
Popis:	A novel antifungal peptide, LAMP-1a, was isolated from sand-elymus (Leymus arenarius) seeds. Expression of a synthetic gene encoding this peptide in Escherichia coli cells was obtained. The target peptide was expressed as a fusion with thioredoxin. Identity of the recombinant peptide to native LAMP-1a was confirmed by chromatography, mass spectrometry, and amino acid sequencing. LAMP-1a displayed a high inhibitory activity in respect of a number of phytopathogenic fungi in in vitro assays, which opens up possibilities for the gene encoding it to be used for genetic transformation of plants and for engineering pathogen-resistant crops
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f03a7e7fbda6fffbf17a38b89c060652 https://doi.org/10.1134/s1022795410120070 Zobrazit plný text záznamu Full text from SpringerLink