ChemInform Abstract: Complex Target SELEX

Autor:	Sharon T. Cload, Judith M. Healy, Steven M. Shamah
Rok vydání:	2008
Předmět:	Cell membrane medicine.anatomical_structure Biochemistry Oligonucleotide Chemistry Aptamer medicine General Medicine Target protein Receptor Small molecule Systematic evolution of ligands by exponential enrichment Ion channel
Zdroj:	ChemInform. 39
ISSN:	1522-2667 0931-7597
Popis:	Aptamers are non-naturally occurring structured oligonucleotides that may bind to small molecules, peptides, and proteins. Typically, aptamers are generated by an in vitro selection process referred to as SELEX (systematic evolution of ligands by exponential enrichment). Aptamers that bind with high affinity and specificity to proteins that reside on the cell surface have potential utility as therapeutic antagonists, agonists, and diagnostic agents. When the target protein requires the presence of the cell membrane (e.g., G-protein-coupled receptors, ion channels) or a co-receptor to fold properly, it is difficult or impossible to program the SELEX experiment with purified, soluble protein target. Recent advances in which the useful range of SELEX has been extended from comparatively simple purified forms of soluble proteins to complex mixtures of proteins in membrane preparations or in situ on the surfaces of living cells offer the potential to discover aptamers against previously intractable targets. Ad...
Databáze:	OpenAIRE
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