Overexpression of growth hormone genes in transgenic mice shortens free‐running periods in constant light
| Autor: | Andrzej Bartke, James S. Ferraro, J. A. Dorsett, Thomas E. Wagner, Jeung S. Yun |
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| Rok vydání: | 1994 |
| Předmět: |
Genetically modified mouse
medicine.medical_specialty Physiology Period (gene) Transgene Heterologous Biology Endocrinology Physiology (medical) Internal medicine medicine Bovine somatotropin Circadian rhythm Phosphoenolpyruvate carboxykinase Microinjection Ecology Evolution Behavior and Systematics |
| Zdroj: | Biological Rhythm Research. 25:315-328 |
| ISSN: | 1744-4179 0929-1016 |
| DOI: | 10.1080/09291019409360306 |
| Popis: | Transgenic mice were produced by microinjection of male pronuclei with approximately 2.7Kb DNA fragment, containing a metallothionein‐I promoter (MT) or a phosphoenolpyruvate carboxykinase (PEPCK) promoter linked to human growth hormone (hGH) or bovine growth hormone (bGH) structural genes. Transgenic mice from resulting lines have substantial levels of circulating heterologous GH and are much larger than normal mice. Since these animals have reproductive abnormalities, and since reproductive hormones have significant effects on the circadian timing system, experiments were designed to determine whether these animals had altered freerunning periods. Transgenic female mice and their normal female siblings were individually housed in cages with activity wheels and exposed to constant dark (DD) or constant light (LL) for durations exceeding two weeks. Locomotor activity was continuously monitored by computer. The period of the circadian rhythm of locomotor activity and the duration of activity were ... |
| Databáze: | OpenAIRE |
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