Induction of apoptosis in HT-29 human colon adenocarcinoma cells by 13-cis-retinoic acid and vitamin E succinate
| Autor: | Kaedi R. Garvin, Brita Brown, Bronwyn G. Hughes, Kim L. O'Neill, David P. Tomer, Philip M. Scherer, Byron K. Murray |
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| Rok vydání: | 2006 |
| Předmět: |
Vitamin
medicine.medical_specialty Nutrition and Dietetics medicine.drug_class Endocrinology Diabetes and Metabolism Vitamin E medicine.medical_treatment Retinoic acid Caspase 3 Cell cycle Biology Molecular biology chemistry.chemical_compound Endocrinology chemistry Apoptosis Internal medicine medicine Retinoid Fragmentation (cell biology) |
| Zdroj: | Nutrition Research. 26:186-192 |
| ISSN: | 0271-5317 |
| Popis: | Recent studies have shown that retinoids and vitamin E are effective growth inhibitors of numerous cancer cell lines. In the present study, vitamin A derivative 13-cis-retinoic acid and α-tocopherol succinate were administered to HT-29 human colon adenocarcinoma cells individually or in combination for a period of 8 days. Cells treated with individual compounds for 8 days attained 80% of the control population. Cells treated with both compounds reached 25% of the control population after 4 days and less than 1% of the control population after 8 days. After 4 days, treated cells became cytostatic, with more than 50% of the cells detaching by day 8. This synergistic effect was specific only for HT-29 cells as compared with normal human colon cells. Treatment resulted in morphological and biochemical changes indicative of apoptotic cell death, including cell cycle blockage at the G1/S interphase, chromatin condensation, nuclear fragmentation, DNA damage, and caspase 3 activation. |
| Databáze: | OpenAIRE |
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