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Reversed-phase high performance liquid chromatography (HPLC) of o-phthalal-dehyde (OPA) derivatives provides a technique that is readily applicable to the estimation of free amino acids (Turnell and Cooper, 1982; Griffin etal., 1982; Price et al., 1984; Palmer, 1985). It has the advantage over classical ion-exchange chromatography of rapidity, increased sensitivity and the ability to handle small sample sizes, utilizing apparatus which is not dedicated to one technique. In previous reports we have described the application of the procedure to amino acid analysis of plasma specimens from normal individuals (Griffin et al., 1982) and from patients with inborn errors of metabolism (Palmer, 1985). Here we describe its application to the screening of urine samples for suspected amino acid disorders. |
