Osteogenic silver oxide doped mesoporous bioactive glass for controlled release of doxorubicin against bone cancer cell line (MG-63): In vitro and in vivo cytotoxicity evaluation
Autor: | Muhammad Asif Tahir, Ali Bahadur, Muhammad Shoaib, Saima Noreen, Muhammad Saif Ur Rahman, Muhammad Bilal Khan, Tahir Mahmood, Muhammad Yasir |
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Rok vydání: | 2020 |
Předmět: |
Materials science
Simulated body fluid 02 engineering and technology Bone tissue 01 natural sciences law.invention chemistry.chemical_compound law 0103 physical sciences Materials Chemistry medicine Bone regeneration 010302 applied physics Process Chemistry and Technology 021001 nanoscience & nanotechnology Controlled release Surfaces Coatings and Films Electronic Optical and Magnetic Materials medicine.anatomical_structure chemistry Bioactive glass Drug delivery Ceramics and Composites 0210 nano-technology Mesoporous material Silver oxide Nuclear chemistry |
Zdroj: | Ceramics International. 46:10765-10770 |
ISSN: | 0272-8842 |
DOI: | 10.1016/j.ceramint.2020.01.086 |
Popis: | Mesoporous bioactive glass demonstrated valuable biomedical applications especially in the field of bone regeneration and drug delivery. For the treatment of cancer by chemotherapy, controlled drug delivery system is one of the significant methods. In this work, silver oxide doped mesoporous bioactive glass nanoparticles (Ag2O-MBG NPs) were developed for the controlled release of doxorubicin as a model drug. Ag2O-MBG NPs were prepared by using the sol-gel method and employing pluronic 123 as an internal template. The prepared Ag2O-MBG NPs were characterized by energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for composition, shape, morphology, and size. Surface area and pore size were determined by the Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH) method respectively. When immersed in simulated body fluid (SBF), hydroxycarbonate apatite (HCAp) formation was demonstrated as established by Fourier Transform Infrared (FTIR) spectroscopy and X-ray diffraction (XRD). The as-synthesized Ag2O-MBG NPs did not show any detrimental effects during MTT assay and in vivo tissue histopathology. Doxorubicin (DOX) was encapsulated with an efficiency of 84% and its release was observed to be affected by the drug loading concentration (0.2–1.0 mg/mL) and pH (6.4–8.4) of the release media. DOX release was of 93% was witnessed approximately for two weeks at a slight acidic pH of 6.4. At 11.88 μg/mL of DOX-Ag2O-MBG NPs, notable inhibitory effects on the viability of the MG-63 osteosarcoma cancer cells were observed. These features proved that the Ag2O-MBG NPs system is effective for bone tissue regeneration and bone cancer treatment. |
Databáze: | OpenAIRE |
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