Phenotypic determination of methicillin-resistant Staphylococcus aureus in Aminu Kano Teaching Hospital, Kano, Nigeria
| Autor: | Yusuf Mohammed, Abdulhadi Sale Kumurya, Abdulrazak Muhammad Idris, Huda Munir Mustapha |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Susceptibility testing business.industry medicine.drug_class Antibiotics biochemical phenomena metabolism and nutrition bacterial infections and mycoses medicine.disease_cause Methicillin-resistant Staphylococcus aureus Methicillin resistance Teaching hospital Age groups Staphylococcus aureus Internal medicine medicine Cefoxitin business medicine.drug |
| Zdroj: | Nigerian Journal of Experimental and Clinical Biosciences. 7:1 |
| ISSN: | 2348-053X |
| DOI: | 10.4103/njecp.njecp_20_18 |
| Popis: | Background: Phenotypic detection of methicillin-resistant Staphylococcus aureus(MRSA) has been problematic ever since its discovery in the early 1960s. The emergence of low-level resistant MRSA clones acquired in the community has only added to these difficulties. In 2005, the Clinical and Laboratory Standards Institute (CLSI) published zone diameter (10), breakpoint guidelines for cefoxitin. However, a number of technical issues remain regarding the use of cefoxitin as a predictor for methicillin resistance. Materials and Methods: In these studies, 252 nonduplicate samples of staphylococcal isolates were collected from various clinical samples obtained from patients attending Aminu Kano Teaching Hospital. The isolates were subcultured and identified using standard bacteriological procedure according to CLSI (13). Antibiotic susceptibility testing was done using a modified form of the Kirby–Bauer method. Methicillin resistance was screened using disk-diffusion method with cefoxitin 30 μg and oxacillin 1 μg. Results: High percentage of the isolates were recovered from patients of age groups of 1–9 years and |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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