Phytochemical Analysis of Secondary Metabolites and Evaluation of the Antioxidant Activity of Centella asiatica (L.) by UV-Vis Spectroscopy
| Autor: | Kaniz Wahida Sultana, Shouvik Chakravarty, Indrani Chandra, Sumanta Das |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Marketing Pharmacology Organizational Behavior and Human Resource Management Antioxidant Centella Traditional medicine biology 010405 organic chemistry Chemistry Strategy and Management medicine.medical_treatment Pharmaceutical Science biology.organism_classification 01 natural sciences 0104 chemical sciences 03 medical and health sciences 030104 developmental biology Ultraviolet visible spectroscopy Phytochemical Drug Discovery medicine |
| Zdroj: | Current Biotechnology. 9:120-126 |
| ISSN: | 2211-5501 |
| DOI: | 10.2174/2211550109999200821155854 |
| Popis: | Background:: Centella asiatica (L.) is an herbaceous, frost-tender perennial plant belonging to the Apiaceae family. It is rich in important secondary metabolites such as terpenoids, flavonoids and saponins. It is used as a traditional medicine for skin infection, cough and asthma. Methods: The present study was intended at the quantitative and qualitative analysis of its secondary metabolites. Thin Layer Chromatography (TLC) was performed to separate the compounds according to their Rf (Retention factor) values. Results:: The total phenolic content of ethanol leaf extract was 45.72 ± 6.05 mg GAE/g determined by Folin Ciocalteu’s assay. The maximum content of total flavonoids was found in aqueous leaf extract, 44.62 ± 2.45 mg QE/g. Aqueous extract of leaf and stem showed the highest antioxidant activity with DPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging assay (45.66 ± 1.29 & 45.20 ± 3.0, respectively). Conclusion:: The present study revealed the antioxidant properties in Centella asiatica. |
| Databáze: | OpenAIRE |
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