Detection of Antibody and the Antigen Classes in Circulating Immune Complexes Fractionated with the Sucrose Density Gradient Centrifugation Using an F(ab')2anti-C3 ELISA

Autor:	Makoto Muratsugu
Rok vydání:	2005
Předmět:	Hepatitis Cirrhosis biology Health Toxicology and Mutagenesis Hepatitis B Toxicology medicine.disease Molecular biology Immune complex Primary biliary cirrhosis Immune system Antigen medicine biology.protein Antibody
Zdroj:	Journal of Health Science. 51:584-589
ISSN:	1347-5207 1344-9702
DOI:	10.1248/jhs.51.584
Popis:	Circulating immune complexes (CICs) in sera from patients with liver cirrhosis (LC), primary biliary cirrhosis (PBC), hepatitis B type (BCH) and hepatitis not including BCH (nonBCH) were analyzed using F(ab′)2anti-C3 ELISA, since CICs’ functions are intimately associated with the composition and their sizes. Positive percentages for IgG-, IgAand IgM-CIC were significantly increased in LC, PBC, BCH and nonBCH as compared to healthy specimens. The combination of F(ab′)2anti-C3 ELISA and sucrose density gradient fractionation allowed us to detect the classes of antibodies and hepatitis B surface antigens in the fractionated CICs from sera of patients with liver diseases. Properties of the fractionized CICs are discussed in the text.
Databáze:	OpenAIRE
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