| Popis: |
Currently, there is no standardised method for making nanomaterials or evaluating their toxicity. Studies show great variability and lack of reproducibility in results, with the discrepancies being caused due to assays used to evaluate exposure toxicity. While the traditional biochemical methods for evaluating toxicity have been employed, there is an issue of how the nanomaterials themselves interact with the dyes, which has created some issues. The current methodologies need to be evaluated to determine the toxicity of the nanomaterials and ensure that there is no interaction between the nanomaterials and the reagents used to complete the assays. This study evaluated the mechanisms of nanomaterial toxicity using mitochondrial function, loss of mitochondrial membrane potential and formation of reactive oxygen species as indicators of toxicity, and compared the data obtained on the BD Pathway 435 confocal to data from a plate reader. Furthermore, nanoparticle uptake and localisation were evaluated using th... |
