PENGARUH PENGAWET BEKU (CRYOPRESERVATION) TERHADAP KADAR EPIDERMAL GROWTH FACTOR (EGF) PADA SELAPUT AMNION

Autor:	Gatut Suhendro, Gunawan Effendi, Indro Handojo, Ety Retno S
Rok vydání:	2018
Předmět:	Andrology chemistry.chemical_compound Membrane chemistry Epidermal growth factor Immunology Glycerol General Earth and Planetary Sciences Biology Elisa method Cryopreservation General Environmental Science
Zdroj:	INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY. 15:22-26
ISSN:	2477-4685 0854-4263
Popis:	to measure the difference of EgF concentration in between fresh amniotic membranes and with cryopreservation in 16 amnioticmembranes. Each amniotic membrane was divided into two parts. the first part was extracted in fresh forms and the second partunderwent cryopreservation with glycerol 50% and was stored at –80° C during 1 month before extraction. Both parts of the membranewere extracted using ultrasonic disintegrator and concentration of EgF was measured from the obtained extract using ELISA method.the average concentration of EgF in the fresh amniotic membrane was 122.76 ± 11.59 pg/g while the average concentration of EgFin the amniotic membrane underwent cryopreservation was 99.34 ± 9.49 pg/g. Average degradation of EgF concentration due tocryopreservation is 18.49% ± 10.20%. EgF concentration in fresh amniotic membrane is significantly higher than the EgF concentrationin amniotic membrane underwent cryopreservation (p = 0.000). Degradation of EgF concentration due to cryopreservation at 95%fidence interval is 12.33% to 24.66%
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::efbd37006259f3d6937177249fc12a3a https://doi.org/10.24293/ijcpml.v15i1.953 Zobrazit plný text záznamu