Deletion of the Intercistronic Poly(A) Tract from Brome Mosaic Virus RNA 3 by Ribonuclease H and Its Restoration in Progeny of the Religated RNA 3

Autor:	L. G. Tyulkina, Olga Karpova, Joseph Atabekov, K. J. Atabekov, Nina Rodionova
Rok vydání:	1989
Předmět:	biology viruses food and beverages RNA RNA-dependent RNA polymerase Nuclease protection assay biology.organism_classification Non-coding RNA Virology Molecular biology Brome mosaic virus RNA editing biology.protein RNase H RNA ligase
Zdroj:	Journal of General Virology. 70:2287-2297
ISSN:	1465-2099 0022-1317
Popis:	Summary The dicistronic genomic RNA 3 of brome mosaic virus (BMV) was used in experiments on site-specific cleavage by RNase H and subsequent religation of large BMV RNA 3 fragments with T4 RNA ligase. BMV RNA 3 was cleaved at the intercistronic poly(A) tract into two fragments: a long (L-BMV 3) 5′-terminal fragment (M r 0.40 × 106) containing the 3a gene, and a short (Sh-BMV 3) fragment (M r 0.28 × 106) containing the coat protein gene and the 3′-terminal tRNA-like tyrosine-accepting structure. Two or three adenylate residues were present at the 5′ end of Sh-BMV 3 and one adenylate at the 3′ end of L-BMV 3. After religation of these RNA fragments BMV RNA 3 was constructed with a deletion including the entire intercistronic poly(A) tract but not the flanking sequences. The religated RNA 3 replicated in wheat plants co-inoculated with BMV RNA 1 and RNA 2. The normal poly(A) tract was restored in progeny BMV RNA 3 during the course of replication.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::efbc34b6a53e23a2b9bea1baaf65ea15 https://doi.org/10.1099/0022-1317-70-9-2287 Zobrazit plný text záznamu