A nanostructured electrochemical aptasensor for highly sensitive detection of chlorpyrifos
| Autor: | Wenjie Hou, Yemin Guo, Jiayun Fu, Jiao Yancui, Xia Sun, Jing Zhao, Xiangyou Wang |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Nanocomposite Materials science Graphene Aptamer 010401 analytical chemistry Metals and Alloys Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Linear range law Specific surface area Electrode Materials Chemistry Electrical and Electronic Engineering 0210 nano-technology Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 243:1164-1170 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2016.12.106 |
| Popis: | A sensitive and selective electrochemical aptasensor based on a novel composite film consisting of carbon black (CB) and graphene oxide@Fe3O4was developed for detection of chlorpyrifos. Chitosan (CS) functionalized CB has high specific surface area, ideal dispersibility and good electrical conductivity, thus, we used them to capture more graphene oxide@Fe3O4 and further amplify the current signal of the aptasensor. The graphene oxide@Fe3O4 nanocomposite formed a unique sensing film with strong synergistic effects, the graphene oxide provided a large surface area, whereas Fe3O4 was uniformly deposited on the graphene oxide, facilitating electron transfer for sensitive detection of chlorpyrifos with excellent selectivity and a low detection limit. Immobilization of the probe Apt on the electrode was greatly improved owing to the unique synergistic effects of graphene oxide@Fe3O4. Therefore, the integration of graphene oxide@Fe3O4 nanocomposite with biomolecules chlorpyrifos aptamer (Apt) as a biorecognition element constructed a novel biosensing platform. Under the optimized conditions, the proposed aptasensor provided a linear range of 0.1–105 ng/mL with a low detection limit of 0.033 ng/mL. The aptasensor performed good selectivity, reproducibility and stability. Additionally, the proposed aptasensor can be applied to monitor chlorpyrifos residues in real vegetable samples. |
| Databáze: | OpenAIRE |
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