Influence of moderate electric field on inactivation kinetics of peroxidase and polyphenol oxidase and on phenolic compounds of sugarcane juice treated by ohmic heating
| Autor: | Bethania Brochier, Ligia Damasceno Ferreira Marczak, Giovana Domeneghini Mercali |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
biology Flavonoid food and beverages 04 agricultural and veterinary sciences 040401 food science Polyphenol oxidase law.invention 0404 agricultural biotechnology Enzyme Biochemistry Magazine chemistry law biology.protein Degradation (geology) Food science Thermolabile Joule heating Food Science Peroxidase |
| Zdroj: | LWT. 74:396-403 |
| ISSN: | 0023-6438 |
| Popis: | This study investigated the non-thermal effects of electricity on enzymatic inactivation kinetics of peroxidase and polyphenol oxidase and on degradation of total phenolic and total flavonoid compounds during ohmic heating of sugarcane juice. A kinetic modeling was carried out from the adjustment of various models applicable to enzyme inactivation, and the Weibull distribution model was the most suitable to describe the inactivation of peroxidase. The presence of an electric field with low intensity significantly influenced the biochemical reactions occurred during peroxidase activation at 60 °C and inactivation at 80 °C. Polyphenol oxidase, a more thermolabile enzyme, was almost totally inactivated before reaching the target temperature (holding phase) of the experiments. The maximum total phenolic and total flavonoid degradation were approximately 23 and 39%, respectively. Statistical analyses showed no differences between conventional and ohmic heating for total phenolic and total flavonoid compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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