Profiling of Nondigestible Carbohydrate Products in a Complete Set of Alien Monosomic Addition Lines Explains Genetic Controls of Its Metabolisms in Allium cepa

Autor: Toshihisa Sumi, Shigenori Yaguchi, Masayoshi Shigyo, Tetsuya Nakajima, Naoki Yamauchi
Rok vydání: 2009
Předmět:
Zdroj: Journal of the American Society for Horticultural Science. 134:521-528
ISSN: 2327-9788
0003-1062
DOI: 10.21273/jashs.134.5.521
Popis: Eight kinds of japanese bunching onion [Allium fistulosum L. (genomes FF)]-shallot [Allium cepa L. Aggregatum group (genomes AA)] monosomic addition line [MAL (FF+1A-FF+8A)] were used to study the effects of single alien chromosomes from A. cepa on the production of carbohydrates in the leaf tissues of A. fistulosum. Carbohydrate contents in green leaf blades of these MALs were measured during alternate months from May 2005 to Mar. 2006. The determination of soluble sugar content from leaf blades of each MAL and A. fistulosum revealed that nonreducing sugars (sucrose and fructan) accumulated in winter leaf blades. Reducing sugar (fructose) in the leaf blades of each MAL was lower than A. fistulosum in almost every time period. In the leaf blades of FF+4A, high fructan accumulation was observed from Nov. 2005 to Mar. 2006. A series of determinations on the pectin content showed that amounts of NaOH-soluble pectin and HCl-soluble pectin remained at low levels at all time periods. High pectin accumulations in FF+7A and FF+8A occurred in September and slightly decreased in November. A decrease in hexametaphosphoric acid-soluble pectin content was associated with the maturity of MALs from autumn to winter, whereas the water-soluble pectin content increased. The pectin methylesterase and polygalactronase genes of shallot were assigned to chromosome 7A and 4A, respectively. These results demonstrate that important genes related to pectin metabolism in shallot are located on chromosomes 4A, 7A, and 8A of shallot.
Databáze: OpenAIRE
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