Human zinc fingers as building blocks in the construction of artificial transcription factors

Autor:	Dong Ki Lee, Young-Do Kwon, Hyun Chul Shin, Kwang-Hee Bae, Kyung-Soon Park, Hyun-Won Kim, Jinwoo Park, Soon Hyung Sohn, Eun-Hyun Ryu, Jin-Soo Kim, Yangsoon Lee, Heung Sun Kwon, Wongi Seol, Byung-Il Yeh, Hyo-Young Yang, Moon-Sun Hwang, Joonho Yoon, Hyean-Woo Lee
Rok vydání:	2003
Předmět:	Zinc finger Genetics Biomedical Engineering Artificial transcription factor Repressor Bioengineering Computational biology Biology Applied Microbiology and Biotechnology DNA-binding protein Fusion protein Molecular Medicine Human genome Gene Transcription factor Biotechnology
Zdroj:	Nature Biotechnology. 21:275-280
ISSN:	1546-1696 1087-0156
DOI:	10.1038/nbt796
Popis:	We describe methods for generating artificial transcription factors capable of up- or downregulating the expression of genes whose promoter regions contain the target DNA sequences. To accomplish this, we screened zinc fingers derived from sequences in the human genome and isolated 56 zinc fingers with diverse DNA-binding specificities. We used these zinc fingers as modular building blocks in the construction of novel, sequence-specific DNA-binding proteins. Fusion of these zinc-finger proteins with either a transcriptional activation or repression domain yielded potent transcriptional activators or repressors, respectively. These results show that the human genome encodes zinc fingers with diverse DNA-binding specificities and that these domains can be used to design sequence-specific DNA-binding proteins and artificial transcription factors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ef772b46264592c969b5d52c7edaa100 https://doi.org/10.1038/nbt796 Zobrazit plný text záznamu Plný text ve formátu PDF