Antigen-dependent fluorescence response of anti-c-Myc Quenchbody studied by molecular dynamics simulations
| Autor: | Hisashi Okumura, Takayoshi Watanabe, Takahiro Hohsaka, Yoshiharu Mori |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Fluorophore
010304 chemical physics biology Metadynamics General Physics and Astronomy 010402 general chemistry 01 natural sciences Peptide antigen 0104 chemical sciences chemistry.chemical_compound Molecular dynamics chemistry Antigen 0103 physical sciences Biophysics biology.protein Physical and Theoretical Chemistry Antibody Fluorescence response |
| Zdroj: | Chemical Physics Letters. 698:223-226 |
| ISSN: | 0009-2614 |
| DOI: | 10.1016/j.cplett.2018.03.011 |
| Popis: | We performed metadynamics molecular dynamics simulations to reveal mechanism of antigen-dependent fluorescence response observed for site-specifically fluorescent-labeled single-chain antibody against c-Myc peptide antigen. We found that V H and V L bind with each other only when the antigen exists and that the fluorophore labeled at the N-terminus of V H interacts with Trp103 most stably. These results support the mechanism proposed from previous experiments: In the absence of antigen, Trp residues are partially exposed at the interface of V H and quench the fluorophore. In the presence of antigen, the Trp residues are buried between V H and V L , and the quenching is eliminated. |
| Databáze: | OpenAIRE |
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