| Autor: |
Ashraf Oukasha Abd El-latif, Ahmed M. A. Salman, Nessma Mohieldeen |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Journal of Plant Protection and Pathology. 11:421-426 |
| ISSN: |
2090-3758 |
| DOI: |
10.21608/jppp.2020.114589 |
| Popis: |
Insect α-amylase has recently become a target of insect control strategies using plant-derived α-amylase inhibitors especially for the control of stored product insect pests. The first step to establish such strategy is to characterize the α-amylase enzymes in the target pest. In the current study, α-amylase was biochemically characterized in the larval and adult stages of Tribolium castaneum and Callosobruchus maculatus. T. castaneum was found to have higher α-amylase activity with lower temperature stability compared to that of C. maculatus. The optimum pH for α-amylase activity was 5-6. Zymogram pattern revealed the presence of two α-amylase isoforms with high molecule weight in T. castaneum. C. maculatus has two α-amylase isoforms in the adult stage and three isoforms in the larval stage. Rice α-amylase inhibitors was purified and found to have strong inhibitory effect against α-amylase of both insects and also negatively affected their life parameters. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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