Serendipitous discovery of Chl d formation from Chl a with papain

Autor:	Y. Itoh, Yoshihiro Shiraiwa, Hajime Koizumi, Takayuki Hoshino, Sadamasa Hosoda, Masami Kobayashi, Machiko Akiyama
Rok vydání:	2005
Předmět:	Pheophytin Chlorophyll a biology Chemistry Acaryochloris marina Chlorophyll d food and beverages macromolecular substances biology.organism_classification Photosynthesis Photochemistry chemistry.chemical_compound Papain Chlorophyll polycyclic compounds General Materials Science Photosensitizer
Zdroj:	Science and Technology of Advanced Materials. 6:551-557
ISSN:	1878-5514 1468-6996
DOI:	10.1016/j.stam.2005.06.022
Popis:	Cancer photodynamic therapy (PDT) requires the availability of photosensitizers which have a high efficiency and selectivity for the destruction of tumor cells. Chlorophyll (Chl) a is one of the favorable photosensitizers, because it has a high extinction coefficient in the red light region, where light transmission through the human tissues is very high. However, Chl a had a serious problem that it cannot be dissolved in water, so we tried to prepare water-soluble chlorophyllide a from Chl a by several enzymes, and serendipitously came across a unique formation of Chl d from Chl a when papain was used in aqueous acetone. Similar oxidation was observed in Chl b and pheophytin a, although the reactions were very slow. Our finding will provide insight into the unsolved key question as to the biosynthetic pathway of Chl d via Chl a in a recently found novel cyanobacterium Acaryochloris marina.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ef45661354ccb33c2262af7ab478903a https://doi.org/10.1016/j.stam.2005.06.022 Zobrazit plný text záznamu