Resolution and contrast enhancement on biological specimens by means of electron spectroscopic imaging (ESI)

Autor:	W. I. Miller, U. B. Hezel, R. Bauer, E. Zellmann
Rok vydání:	1986
Předmět:	Biological specimen Nuclear magnetic resonance Contrast enhancement Materials science Resolution (electron density) General Medicine Electron
Zdroj:	Proceedings, annual meeting, Electron Microscopy Society of America. 44:68-69
ISSN:	2690-1315 0424-8201
DOI:	10.1017/s0424820100142049
Popis:	The main elemental constituents of biological material - C,H,N,O - are the same elements found in typical embedding materials. Because of this the contrast of unstained biological material is very poor. Additionally, electron scattering by low Z atoms is mainly inelastic resulting in unsharp images from the concomitant chromatic aberration.These effects have been delt with by employing stains of such heavy metals as Os, U, or Pb. These stains are for the most part located at the biological structures themselves and primarily scatter the electron beam elastically. Thus with ultra-thin (
Databáze:	OpenAIRE
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