Biomimetic in situ nucleation of calcium phosphates by protein immobilization on high strength ceramic materials
| Autor: | Jennifer Spitz, Rainer Telle, Karolina Schickle, Sabine Neuss |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
biology Scanning electron microscope Simulated body fluid Nucleation 030206 dentistry 02 engineering and technology 021001 nanoscience & nanotechnology 03 medical and health sciences 0302 clinical medicine Chemical engineering visual_art Materials Chemistry Ceramics and Composites visual_art.visual_art_medium biology.protein Surface modification Cubic zirconia Cap formation Ceramic Composite material Bovine serum albumin 0210 nano-technology |
| Zdroj: | Journal of the European Ceramic Society. 38:271-277 |
| ISSN: | 0955-2219 |
| Popis: | Zirconia has been commonly used in the dental industry because of its excellent biological, mechanical and aesthetic properties. This material, however, is classified as nearly inert. To bioactivate the ceramic surface, biomimetic depositions of calcium phosphate coatings have been developed. We demonstrate an accelerated biomimetic coating method on zirconia using a specific pre-treatment with biological agents. We have chosen bovine serum albumin as a standard protein. Through the pre-treatment of the zirconia using a hydroxylation or additionally immobilizing the bovine serum albumin on the surface, we could influence the CaP deposition rate. Immunohistochemical analyses verified the presence of BSA on the zirconia surfaces. After immersion in simulated body fluid at 40 °C, the samples were analyzed by scanning electron microscopy and X-ray diffraction to visualize the CaP formation. Here we could show as proof-of-principle that it is possible to accelerate biomimetic coating processes on zirconia implants containing BSA on their surface. |
| Databáze: | OpenAIRE |
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