Immunomodulation of human CD19+CD25high regulatory B cells via Th17/Foxp3 regulatory T cells and Th1/Th2 cytokines
| Autor: | Guoqing Pan, Deyu Kong, Jin Liang, Min Hong, Change Gao, Weiqing Liu, Jun Deng, Jie Zhang, Zhaoming Zhong, Yun Liao, Xiao Chen, Shaoyou Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology Chemistry Regulatory B cells medicine.medical_treatment Immunology FOXP3 General Medicine In vitro CD19 Cell biology Proinflammatory cytokine 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Cytokine medicine biology.protein Immunology and Allergy Tumor necrosis factor alpha 030215 immunology Transforming growth factor |
| Zdroj: | Human Immunology. 80:863-870 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/j.humimm.2019.05.011 |
| Popis: | Regulatory B (Breg) cells are a special subset of immunoregulatory cells with unique phenotypes and functions. In this study, human CD19+CD25high Breg cells were purified from human peripheral blood. Based on the coculture system of Breg cells and CD4+ T cells in vitro, Breg cells were found to promote the increase in regulatory T (Treg) cells while decreasing the number of Th17 cells. Breg cells regulate Treg cells through two processes: cell-cell contact and cytokines. TGF-βsRII, a blocker of transforming growth factor-β (TGF-β), can attenuate the effects of Treg elevation, suggesting that TGF-β is the main cytokine, while Breg cells rather than interleukin-10 (IL-10) regulate the differentiation of Treg cells. However, Th17 cells were mainly regulated by cytokines, without an obvious regulatory effect on cell-cell contacts. Breg cells may regulate Th17 cells by a pathway independent of TGF-β and IL-6. The coculture of Breg cells and CD4+ T cells led to changes in the cytokine spectrum, which included significant increases in IL-4, IL-6 and IL-10 but not obvious changes in IL-2, IFN-γ and TNF. The inhibitory effect of Breg cells was weakened by blocking cell-cell contacts in cultures separated with the Transwell chamber because IL-10 decreased while IL-6 increased when compared with cocultured Breg and CD4+ T cells. When the IL-10 inhibitor IL-10sRα was added, IL-6 and TNF levels significantly increased, while treatment with the TGF-β inhibitor TGF-βsRII did not result in similar changes, suggesting that IL-10 is an important molecule to inhibit the proinflammatory factors IL-6 and TNF in this culture system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect