Immobilized-enzyme membrane sandwich reactor used in automated micro-scale assay of plasma uric acid

Autor:	P Kamoun, O Douay
Rok vydání:	1978
Předmět:	Chromatography Immobilized enzyme biology Biochemistry (medical) Clinical Biochemistry Inorganic chemistry Plasma Fluorescence chemistry.chemical_compound Membrane chemistry biology.protein Molecule Uric acid Hydrogen peroxide Peroxidase
Zdroj:	Clinical Chemistry. 24:2033-2035
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/24.11.2033
Popis:	We describe the automated microassay of plasma uric acid by use of an immobilized uricase-membrane sandwich reactor. Hydrogen peroxide, formed when uric acid is oxidized, oxidatively couples two molecules of p-hydroxyphenylacetic acid in the presence of peroxidase to produce a highly fluorescent compound. The specificity of the uricase reaction is coupled with a significantly lower cost of analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ef21f8c16aaadb81a3f48f87299f0146 https://doi.org/10.1093/clinchem/24.11.2033 Zobrazit plný text záznamu