An efficient nucleic acids extraction protocol for Elettaria cardamomum
| Autor: | Jebasingh Tennyson, Sankara Naynar Palani, Aathira Menon, Sangeetha Elangovan, Manoharan Kumariah |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Small RNA food.ingredient Elettaria cardamomum RNA Bioengineering Biology Ascorbic acid 01 natural sciences Applied Microbiology and Biotechnology chemistry.chemical_compound food chemistry Biochemistry 010608 biotechnology Gene expression Nucleic acid RNA extraction Agronomy and Crop Science DNA 010606 plant biology & botany Food Science Biotechnology |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 17:207-212 |
| ISSN: | 1878-8181 |
| Popis: | Elettaria cardamomum is an economically important spice crop. Genomic analysis of cardamom is often faced with the limitation of inefficient nucleic acid extraction due to its high content of polyphenols and polysaccharides. In this study, a highly efficient DNA and RNA extraction protocol for cryopreserved samples from small cardamom plant was developed with modification in the CTAB and SDS method for DNA and RNA, respectively, with the inclusion of 2% ascorbic acid. DNA isolated by this method is highly suitable for PCR, restriction digestion and RAPD analysis. The RNA extraction method described here represent the presence of plant mRNA, small RNAs and viral RNA and, the isolated RNA proved amenable for RT-PCR and amplification of small and viral RNA. Nucleic acids extraction protocol developed here will be useful to develop genetic marker for cardamom, to clone cardamom genes, small RNAs and cardamom infecting viral genes and to perform gene expression and small RNA analysis. |
| Databáze: | OpenAIRE |
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