Do Catecholamines Play a Physiologic Role in Regulating Corpus Luteum Function in the Pseudopregnant Rabbit?1
| Autor: | P L Keyes, John E. Gadsby, T S Schwartz, B Lucchesi, Charles H. Bill |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class medicine.medical_treatment Cell Biology General Medicine Propranolol Biology Luteal phase Receptor antagonist Effective dose (pharmacology) Endocrinology medicine.anatomical_structure Reproductive Medicine In vivo Internal medicine Luteolysis medicine Saline Corpus luteum medicine.drug |
| Zdroj: | Biology of Reproduction. 32:907-915 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod32.4.907 |
| Popis: | In these studies the beta-adrenergic receptor antagonist propranolol was administered to estrogen-treated hypophysectomized pseudopregnant rabbits in vivo, and serum progesterone concentrations were measured to monitor luteal function. In Experiment 1, which was designed to determine an effective dose of propranolol, 1 mg/(kg X h) s.c. propranolol for 3 h (integral of 80 ng/ml in serum) gave an adequate level of beta-adrenergic receptor blockade, i.e., a 1000-fold inhibition of the blood pressure/isoproterenol dose-response relationship. In Experiment 2, "acute" administration of propranolol (P; 1 mg/(kg X h) s.c.) or saline (control, C) for 24 h on Days 7-8, 10-11, and 13-14 of pseudopregnancy did not produce any marked differences in serum progesterone concentrations in P or C animals on any of the days tested, although hourly fluctuations were observed. In Experiment 3, "chronic" (4-day) treatment with propranolol was achieved by the use of propranolol-containing pellets placed s.c. (integral of 200-600 ng/ml in serum), on Days 13-17. Control animals received pellets of vehicle only. Serum progesterone concentrations were very similar in P and C animals throughout the period of treatment (Days 13-17) and on Days 18 and 20. We conclude that endogenous catecholamines play no major role in regulating luteal steroidogenesis or corpus luteum regression in the pseudopregnant rabbit. |
| Databáze: | OpenAIRE |
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