Determination of the Origin of 19-Norandrosterone in Urine by Gas Chromatography–Isotope-Ratio Mass Spectrometry for Doping Control
| Autor: | T. G. Sobolevskii, M. A. Dikunets, I. I. Podolskiy |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromatography 010401 analytical chemistry Extraction (chemistry) Mass spectrometry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Isotope fractionation chemistry Sample preparation Gas chromatography Isotope-ratio mass spectrometry 19-Norandrosterone |
| Zdroj: | Journal of Analytical Chemistry. 73:283-291 |
| ISSN: | 1608-3199 1061-9348 |
| DOI: | 10.1134/s1061934818010082 |
| Popis: | The most difficult task in antidoping control is the identification of compounds whose origin can be either endogenous or exogenous. This paper presents a procedure for determining the origin of 19-norandrosterone based on the extraction of target components from urine, their multistage purification by semipreparative HPLC, acetylation, and quantification by gas chromatography–isotope-ratio mass spectrometry and gas chromatography–mass spectrometry. Effects of isotope fractionation were not observed in sample preparation. The degree of extraction of the target analytes was 80 ± 5%. |
| Databáze: | OpenAIRE |
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