Establishment, characteristics, and diagnostic uses of cell cultures derived from tissues of exotic species
| Autor: | Jill F. Dotson, Anthony E. Castro |
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| Rok vydání: | 1988 |
| Předmět: | |
| Zdroj: | Journal of Tissue Culture Methods. 11:113-121 |
| ISSN: | 1573-0603 0271-8057 |
| DOI: | 10.1007/bf01404263 |
| Popis: | Methods for the preparation, cultivation, preservation, and maintenance of serially passaged cell cultures derived from different tissues of exotic or threatened animals and certain species of birds are described. The diagnoses of viral pathogens affecting exotic and domestic species were enhanced by the availability of such cell or avian tracheal ring organ cultures to the veterinary diagnostic virology laboratory. Cell cultures from exotic species derived from tissues of bighorn sheep, white-tailed gnu, white-tailed deer, oryx, aoudad sheep, cockatiels, and ring-necked pheasants were developed or obtained by our laboratory. These cell cultures were examined for cellular morphology and growth characteristics at two different temperatures, presence of adventitious agents, and karyotype. Several of the exotic cell lines supported the replication of various viruses associated with avian and mammalian hosts. As comparison, cell cultures of domestic sheep were used. Tracheal ring organ cultures from chickens, turkeys, and pheasants were also assayed for their sensitivity to selected viruses. Cell cultures from exotic species may provide a viable alternative for the detection of viruses from such species which heretofore have been difficult to isolate using cell lines derived from domestic species. |
| Databáze: | OpenAIRE |
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