| Autor: |
A. W. Neumann, Frank Y H Lin, H.K. Yueh, Z. Policova, E. Moy |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Colloids and Surfaces B: Biointerfaces. 1:23-32 |
| ISSN: |
0927-7765 |
| DOI: |
10.1016/0927-7765(93)80014-p |
| Popis: |
The surface tensions of human albumin, immunoglobulin G, and fibrinogen were determined by using two different techniques: (1) the freezing front technique and (2) the pendant drop arrangement in conjunction with axisymmetric drop shape analysis-profile (ADSA-P). The changes in surface tension, resulting from the adsorption of proteins from solution, at various concentrations, onto polymer particles are reported by using the freezing front technique. Furthermore, the adsorption of proteins onto the solution—air interface is also reported by using the pendant drop arrangement. The large differences between the two types of measurements are discussed in terms of the different extents of conformational changes in response to different interfacial tensions driving these changes. A further qualitative difference between the two methods as used here is that the ADSA surface tension measurement registers only the properties of the top few angstroms of the layer whereas the solidification front technique registers the presence of much thicker layers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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