Genotyping Giardia intestinalis by Using DNA Extracted from Long-Term Preserved Human Specimens Stained with Chlorazol Black E
| Autor: | Norihito Morimoto, Masataka Korenaga, Yutaka Komatsu, Tetsuro Sugiura, Hiroaki Takeuchi, Yoshihisa Matsumura, Yoshie Nishida |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030231 tropical medicine Giardia General Medicine 030108 mycology & parasitology Biology biology.organism_classification Giardia Infections Microbiology 03 medical and health sciences Diarrhea 0302 clinical medicine Infectious Diseases parasitic diseases Genotype medicine Restriction fragment length polymorphism medicine.symptom Pathogen Genotyping Feces |
| Zdroj: | Japanese Journal of Infectious Diseases. 69:244-247 |
| ISSN: | 1884-2836 1344-6304 |
| Popis: | Giardia intestinalis is a parasitic protozoan that causes diarrhea and abdominal pain in humans. Studies of the Giardia genotypes are thought to be important for understanding their infection routes and prevalence. However, few have reported pathogen genotyping in human giardiasis cases in Japan. In this study, we genotyped G. intestinalis by using DNA extracted from chlorazol black E-stained fecal smears from patients. The triosephosphate isomerase gene was amplified from 21 (91.3%) of 23 human fecal samples. Twelve (52.2%) of pathogens detected were of the genotype A, and 9 (39.1%) of the genotype B. A restriction fragment length polymorphism analysis showed that all genotype A found in the present study were of the genotype AI, which were presumed to be zoonotic. The source of Giardia infections was unclear in the present study. However, patients' histories of international travel appeared not to be associated with the Giardia genotypes. Thus, most cases were thought to be acquired sporadically and domestically. |
| Databáze: | OpenAIRE |
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