Autocrine regulation of gonadotropin‐releasing hormone (GnRH) operates at multiple control levels of GnRH gene expression in GT1–1 neuronal cells
| Autor: | Jin Han, Kyungjin Kim, Sehyung Cho, Woong Sun |
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| Rok vydání: | 1998 |
| Předmět: |
Agonist
endocrine system medicine.medical_specialty Chemistry medicine.drug_class Gonadotropin-releasing hormone Inhibitory postsynaptic potential Buserelin Incubation period Endocrinology Internal medicine Gene expression medicine Autocrine signalling hormones hormone substitutes and hormone antagonists medicine.drug Hormone |
| Zdroj: | Korean Journal of Biological Sciences. 2:483-488 |
| ISSN: | 1226-5071 |
| Popis: | We previously found that a potent gonadotropin‐releasing hormone (GnRH) agonist, buserelin, decreases GnRH promoter activity together with GnRH mRNA level, providing evidence for an autoregulatory mechanism operating at the level of GnRH gene transcription in immortalized GT1–1 neuronal cells. To examine whether agonist‐induced decrease in GnRH mRNA level requires the continuous presence of buserelin, we performed a pulse‐chase experiment of buserelin treatment. Short‐term exposure (15min) of GT1–1 neuronal cells to buserelin (10 μM) was able to decrease GnRH mRNA levels when determined 24 h later. When GT1–1 cells were treated with buserelin (10 uM) for 30min and then incubated for 1, 3, 6, 12, 24, and 48 h after buserelin removal, a significant decrease in GnRH mRNA levels was observed after the 12 h incubation period. These data indicate that inhibitory signaling upon buserelin treatment may occur rapidly, but requires a long time (at least 12 h) to significantly decrease the GnRH mRNA level. To examin... |
| Databáze: | OpenAIRE |
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