SHE Cell Transformation Assay as a Predictive System for Carcinogenicity of PAH and Non-Genotoxic Carcinogens

Autor:	Halima Bessi, G Nguyen-Ba, C. Rast, Paule Vasseur, Stéphanie Alexandre, Marie-José Durand, Véronique Cruciani
Rok vydání:	1996
Předmět:	Polymers and Plastics Stereochemistry Chemistry Organic Chemistry Cell Hamster DMBA Molecular biology In vitro Transformation (genetics) chemistry.chemical_compound medicine.anatomical_structure Benzo(a)pyrene Carcinogen Screening Materials Chemistry medicine Carcinogen
Zdroj:	Polycyclic Aromatic Compounds. 10:307-314
ISSN:	1563-5333 1040-6638
DOI:	10.1080/10406639608034711
Popis:	The Syrian hamster embryo (SHE) cell transformation assay is an in vitro method for the detection of genotoxic and non-genotoxic carcinogens. Its reliability as a multistage carcinogen screening method was demonstrated by several authors who showed that cells derived from transformed colonies were capable of tumour formation when inoculated in a suitable host animal. The SHE assay offers the advantage of using normal diploid cells with metabolizing capacities and low spontaneous transformation frequency. Epithelial cultures can be selected from the mixed cellular suspension obtained from the Syrian hamster embryos in order to develop an in vitro model for the detection of carcinogens inducing carcinomas. Polycyclic aromatic hydrocarbons, such as BaP and DMBA were studied in depth for their cell transforming potency. The synergistic effects of PAH and tumor promoters can be demonstrated by using a sequential treatment consisting in an initiation-promotion scheme1. This protocol also revealed inter...
Databáze:	OpenAIRE
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